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Global Meet on Pharmaceutical Sciences

Zurich, Switzerland

Screening and Potential of Gram Negative Bacterial Isolates for their Extracellular Enzymatic Activities Isolated from the Hospital Aquatic Environment

Author(s): Manzar Alam and Mohd Imran*

Bacteria can resist an antimicrobial agent by producing extracellular enzymes that eradicate or inactivate the antibiotics. Majority of drug/metal resistant Gram negative bacterial isolates showed amylase, β-Lactamase, protease, lipase, gelatinase and urease enzymatic activity. In isolates from site-1, maximum 96%, 74% and 72% of the total isolates showed amylase, lipase and catalase activity, whereas only 46%, 34%, 24% and 20% exhibited β-Lactamase, urease, protease and gelatinase activity respectively. In site-II, 88%, 84%, 74%, 68%, 58% and 56% of the isolates demonstrated lipase, protease, amylase, catalase, gelatinase and urease activity respectively, while only 48% of the isolates showed β-Lactamase activity. In case of site- III, maximum 86%, 74%, 68%, 62%, 56% and 52% of the total isolates exhibited lipase, amylase, β-Lactamase, catalase, urease and protease activity respectively. Whereas, only 46% of the isolates demonstrated gelatinase activity. In case of site-IV, maximum 96%, 80%, 66%, 62% of the isolates showed catalase, protease, β-Lactamase and gelatinase activity respectively. Whereas only 48% and 46% of the isolates demonstrated amylase, lipase and urease activity. In our observation most of the isolates from site I, II, III showing lipase activity its mean the hospital wastewater have been suitable source (carbon, nitrogen, salt and mineral) for growth and production of lipase activity. This result revealed that the presence of multi drug/metal resistance Gram negative bacteria from the hospital wastewater were showing various amylase, protease, lipase, catalase, betalactamase, gelatinase and urease, resistance mechanisms and this drug resistant strains may cause infections to the healthy living things. To reduce the spread of drug/metal resistant isolates from hospital to environment, and use good safety sterilization methods before release of waste materials to the environment or sewage.


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