School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning , Guangxi Province, China


  • Research Article   
    A Novel Nitidine Chloride Nanoparticle Inhibits the Stemness of CD133+EPCAM+ Huh7 Hepatocellular Carcinoma Cells
    Author(s): Danni Li, Qiying Zhang, Yuzhu Zhou, Hua Zhu* and Tong Li*


    Nitidine chloride is a natural product. We synthesized novel nanoparticles of Nitidine chloride (TPGS-FA/NC), which evaluated anti-hepatocellular carcinoma potential capability in vitro and in vivo. Cell viability was assessed by MTT and colony assays; TPGS-FA/NC was examined by Confocal Microscopy though targeting Huh7 Hepatocellular Carcinoma Cells. A sphere culture technique was used to enrich Cancer Stem Cells (CSC) in Huh7 cells. The in vivo antitumor efficacy of TPGS-FA/NC was evaluated in Huh7 cell xenograft model, which were administered by TPGS-FA/NC for 2 weeks. TPGS-FA/NC (10, 20, 40 μg/mL) dose-dependently inhibited the proliferation of HCC cells. Interestingly, TPGS- FA/NC (10, 20, 40 μg/mL) drastically reduced the EpCAM+/CD133+ cell numbers, which suppressed the sphere formation and inhi.. Read More»