Objective: The present study was carried out to investigate the possible in vivo analgesic, neuropharmacological and anti-diarrheal activities of the methanol extract of Jatropha gossypifolia fruits. Materials and Methods: The analgesic activity was measured by acetic acid induced writhing inhibition test. The neuropharmacological activities were evaluated by hole cross, hole-board, and elevated plus-maze (EPM) tests and the anti-diarrheal activity was assessed by castor oil induced diarrhea inhibition method. Findings: The extract showed highly signiicant (P < 0.001) analgesic activity with % inhibitions of writhing response at doses 200 and 400 mg/kg body weight were 77.86% and 71.25%, respectively. The extract at both doses showed signiicant (P < 0.05) sedative effect in-hole cross test. In-hole board test, the extract showed highly signiicant (P < 0.001) anxiolytic activity at lower dose whereas this activity was observed at higher dose in EPM test. The extract also showed highly signiicant (P < 0.001) anti-diarrheal activity. Conclusion: The indings of the study clearly indicate the presence of signiicant analgesic, neuropharmacological and anti-diarrheal properties of the plant, which demands further investigation including, compound isolation.