Magnetized water showed alterations in the electrolyte potential and vibration modes. This study aimed to investigate the effect of magnetic water and physiological solutions on the UV-spectra of tramadol HCl. Distilled water as well as full strength or diluted physiological solutions are magnetized by magnetic disc. Then tramadol HCl was dissolved in each magnetic and in non-magnetic solution to obtain the following final concentrations: 0, 25, 50, 100, and 200μg/mL. The pH of each solution and the absorbance (O.D.) was recorded at wavelength 271 nm. Magnetized water or physiological solutions showed alterations in pH as well as in the absorbance (O.D.) of tramadol HCl detected by ultraviolet spectra at λ271 nm. It concludes that magnetized physiological solutions adversely altered the stability of tramadol HCl.