Objective: Elimination of microorganisms from the root canal system plays a fundamental role in successful endodontic treatment. This study aimed to assess the antibacterial effect of three intra canal medicaments with/without electrophoresis on Enterococcus faecalis (E. faecalis) at different dentin depths.
Materials and methods: This study evaluated 81 sound extracted singlerooted, single-canal human teeth. After root canal preparation, the teeth were sterilized, and E. faecalis was inoculated into the root canals for 2 weeks. The teeth were then randomly divided into 9 groups (n=9) of Calcium Hydroxide (CH) without electrophoresis, CH with electrophoresis, Cupral without electrophoresis, Cupral with electrophoresis, silver nanoparticles (AgNPs) with CH without electrophoresis, AgNPs with CH and with electrophoresis, electrophoresis alone, negative control group, and positive control group. The medicaments were applied in the root canals and the teeth were incubated for 4 weeks. In electrophoresis groups, 15 mA electric current was applied for 6 min, and the teeth were then incubated. Then, dentin chips were collected from three different dentin depths by peeso reamers, and cultured on agar and thioglycolate culture media.
Clinical relevance: Increasing the penetration of intra canal medicaments into the dentin increases their antimicrobial effect.
Results: No significant difference was noted in antimicrobial activity of tested materials at different depths (P>0.05). A significant difference was noted in elimination of microbial biofilm between groups with and without electrophoresis at depths 2 and 3 (P<0.05).
Conclusion: Electrophoresis resulted in greater penetration of medicaments into dentinal tubules, and enhanced their antimicrobial efficacy in deeper areas of dentinal tubules.