Momordica charantia L. belongs to the family Curcubitaceae and it is very common in many Brazilian regions. The plant is a liana with flowers and yellow fruits that present red seeds when are ripe. Popularly known as “melão-de-são-caetano”, “melão amargo” or “cabaço-amargo”, it possesses many uses: antidiabetic, antihelmintic, antmicrobial, anticancerigenous and antioxidant. The phytochemical prospection of the fresh and dried leaves extracts showed the presence of different classes of secondary metabolites, as flavonoids, alkaloids and tannins, that have dem-onstrated antimicrobial action. Fresh and dried leaves presented significantly anti-microbial activity against all bacterial strains tested, specially Escherichia coli. Ethyl acetate fractions were effective against Escherichia coli and Bacillus cereus. The mod-ulatory activity was significative too.