Genotoxic and Hypogonadism Effect of Triclosan Treatment and the Mitigating Effect of Vitamin E in Male Albino Mice

Author(s): Nahed A Hussien* and Hamida Hamdi

The widely used antimicrobial compound, triclosan (TCS), causes various harmful impacts to human. The present study was aimed to evaluate the genotoxic and antiandrogenic potential of TCS represented by DNA damage (Comet assay), histopathological and testicular hormonal evaluations. Moreover, the study was aimed to assess the protector role of vitamin E (Vit E) pre-treatment in testes of albino male mice. TCS was injected IP at dose level (15 mg/kg) for 2 consecutive days. Other group was orally administrated with Vit E (50 mg/kg) just before TCS injection. Mice were sacrificed after 24 hr from the last treatment. Testicular tissues were used for molecular, histopathological evaluations and different testicular hormones (testosterone, FSH and LH) determination. The present study reports the genotoxic effect of TCS because of a significant increase in tail length, %DNA in tail and tail moment for TCS group in comparison with the negative control group in comet assay. Moreover, TCS treatment causes damage to testis architectures represented by the separated seminiferous tubules, pyknotic spermatocytes, vacuolization in seminiferous epithelium due to loss of Sertoli cells and hypospermatogenesis. In addition, TCS treatment significantly decreases testicular hormones (testosterone, FSH and LH) in comparison to negative control group. However, Vit E pre-oral administration reverses the toxic effect of TCS. In conclusion, the present study reports the genotoxic and antiandrogenic effect of TCS in male mice and the ameliorative effect of Vit E pre-oral administration as a result of its ROS scavenger properties

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