Background and Objectives: Diabetes mellitus is a chronic metabolic disorder, which is characterized by alterations in carbohydrates, protein and fats metabolism and have been connected to all age’s people worldwide. Thus, the aim of present research work was to isolate and determine the anti-diabetic activity of compound from the plant P. glaucus (Lam) Merr in STZ-induced diabetic rats. Materials and Methods: The isolation and identification of compound was determined with the help of column chromatography followed by TLC, GCMS and 1HNMR. The anti-diabetic profile of the isolated compound was investigated in STZ- induced diabetic rats at single dose 50 mg/kg (b.wt). The glibenclamide at a dose of 20 mg/kg (b.wt), p. o) was used as a standard. Results and Discussion: The compound benzoic 2-[(trimethylsilyl) oxy]-, methyl ester (cas) methyl o-trim ethyl-silylsalicylate was isolated from active fractionation with the help of column chromatography followed by TLC, GCMS and 1HNMR. The RF value determined on TLC plate was 0.33. The M.wt of isolated compound was 308; H NMR (399.6 MHz); retention time 22.26; mz 209; % area 7.43 and area was 2110418.00. The molecular formula was C21H22O2. The isolated compound significantly (P<0.001) reduced blood sugar level in STZ-induced diabetic rats at single dose 50 mg/kg (b.w) respectively as matched to untreated diabetic control group. The attenuation in blood sugar level with isolated compound was 15.10 ± 0.09 mmol/L at 6 hrs, 13.70 ± 0.09 mmol/L at 12 hrs and 11.15 ± 0.25 mmol/L at 24 hours as matched to untreated diabetic control group. Conclusions: The conclusions of this in vivo experimental study indicate that the compound isolated from P. glaucus possesses significant hypoglycemic properties; and thus provides the justification of P. glaucus in the remedy and as well as in the management of diabetes mellitus.