Introduction: Adverse drug reactions (ADRs) enhance suffering of patients and increase morbidity and mortality. Pharmacovigilance (PV) is an integral part of drug therapy as it concerns with the detection, assessment, understanding and prevention of ADRs. The spontaneous reporting system is the most essential pillar of Pharmacovigilance system. Apart from drug dispensing and compliance, pharmacists can have a substantial role in ADR reporting and significant impact on spontaneous reporting of ADRs. Aims and Objectives: To assess the knowledge, attitude and practice of pharmacovigilance among pharmacists and exploring their participation in ADR reporting system. Methodology: A cross sectional study was conducted among pharmacists of Rajkot district, using a pretested questionnaire (15 questions, 5 questions of each on knowledge, attitude and practice aspects of pharmacovigilance). Descriptive statistics were used to analyse the results. Results: Response rate was 82%. 79.60% participants had bachelor degree of pharmacy and 70.93% were practicing as pharmacists (community pharmacists- 68.93%). Rest were academicians and working professionals. Pharmacists had insufficient knowledge of PV with poor mean knowledge score (mean score 1.46 out of 5). About two-thirds of the pharmacists expressed a positive attitude toward ADRs reporting and agreed that they are willing to participate in reporting of ADRs. Only single case of ADR was reported by pharmacists disclosed that pharmacists were unawareness of such existing ADR reporting system in India. Conclusion: There is need for educational programs/workshop or training on how to report and where to report ADRs and also to emphasize their role and responsibility regards to PV.